Detection of differentially expressed genes in synovial fibroblasts by restriction fragment differential display
نویسندگان
چکیده
منابع مشابه
Detection of differentially expressed genes in synovial fibroblasts by restriction fragment differential display.
OBJECTIVE To identify differentially expressed genes in synovial fibroblasts and examine the effect on gene expression of exposure to TNF-alpha and IL-1beta. METHODS Restriction fragment differential display was used to isolate genes using degenerate primers complementary to the lysophosphatidic acid acyl transferase gene family. Differential gene expression was confirmed by reverse transcrip...
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~The first two authors contributed equally to the establishment of the method and to the results obtained. 4present address: Max-Planck-Gesellschaft, MaxDelbr~ick-Haus, 13122 Berlin-Buch, Germany. Analysis of gene expression is a central aim in most studies in molecular and cell biology. Interest in tissue-specific gene expression and, in particular, in changes in the expression patterns occurr...
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Abbreviations: ARP: acidic ribosomal protein CHS: chalcone synthase DDRT-PCR: differential display reverse transcriptase polymerase chain reaction DETs: differentially expressed transcripts DPA: days post anthesis ESTs: expressed sequence tags MBD: methyl CpG binding domain OPT: oligopeptide transporter protein PCR: polymerase chain reaction PG: polygalacturonase enzyme RRF: ribosome recycling ...
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ژورنال
عنوان ژورنال: Rheumatology
سال: 2004
ISSN: 1462-0332,1462-0324
DOI: 10.1093/rheumatology/keh347